PK-15-CAS9 cell line

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location： Home > Products > Cas9 Stable Cell Lines > PK-15-CAS9
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  • Wild-type Cell Lines-1 PK-15-CAS9-Wild-type Cell Lines-1
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  • Transfection PK-15-CAS9-Transfection
  • PK-15-CAS9 cell line PK-15-CAS9-产品图
Wild-type Cell Lines-1 PK-15-CAS9-Wild-type Cell Lines-1
Wild-type Cell Lines-2 PK-15-CAS9-Wild-type Cell Lines-2
Transfection PK-15-CAS9-Transfection
PK-15-CAS9 cell line PK-15-CAS9-产品图
PK-15-CAS9 Copy Product Info
Catalog#： YC-A017-Cas9-H
Size： 1*10^6
Culture method： 90%DMEM+10%FBS
Datasheet (Use Instruction)
  • Wild-type Cell Lines-1 PK-15-CAS9-Wild-type Cell Lines-1
  • Wild-type Cell Lines-2 PK-15-CAS9-Wild-type Cell Lines-2
  • Transfection PK-15-CAS9-Transfection
  • PK-15-CAS9 cell line PK-15-CAS9-产品图
PK-15-CAS9 Copy Product Info
Catalog#： YC-A017-Cas9-H
Size： 1*10^6
Culture method： 90%DMEM+10%FBS
Datasheet (Use Instruction)

Detailed Product Information

Cell Info

Product category Cas9 Stable Cell Lines Mycoplasma test negative
Cell Morphology Epithelial-like, adherent Culture method 90%DMEM+10%FBS
Passage ratio 1：2-1：4 Species Pig
Expression gene Cas9 Resistance gene hygro
Product category
Cas9 Stable Cell Lines
Mycoplasma test
negative
Cell Morphology
Epithelial-like, adherent
Culture method
90%DMEM+10%FBS
Passage ratio
1：2-1：4
Species
Pig
Expression gene
Cas9
Resistance gene
hygro
Recombinant Cas9 protein derived from Streptococcus pyogenes is a protein that plays a key role in CRISPR/Cas9 system. It has endonuclease activity; Under the guidance of sgRNA, Cas9 protein can move to the target gene site, cut DNA accurately, and cause DNA double-strand breaks. Ubigene's PK-15 cell line was constructed on the basis of wild-type PK-15 cells by lentiviral transduction of the engineered Cas9 protein gene, which can stably express Cas9 protein for a long time. Using this cell, efficient gene knockout, knock-in and point mutation in PK-15 cells can be achieved by only transfecting sgRNA or co-transfecting sgRNA and donor DNA. It can also be used for the construction of cell knockout library and easily solve the problem of Cas9 transfection difficulty.

Product Validation Data

RT-qPCR
Sample Name Target Name Cт Mean ΔCт
PK-15-CAS9 Cas9 21.41878700 4.06616783
PK-15-CAS9 β-actin 17.35261917
PK-15 Cas9 29.50979614 11.11094475
PK-15 β-actin 18.39885139

Product Advantage

  • icon
    Easy to use
    Cas9 cell lines in our cell bank can stably express Cas9 protein. Each Cas9 Stable Cell Line is easy to use and enables gene knockout simply by transfecting gRNA, while transfection of gRNA and donor DNA results in gene knock-in or point mutations
  • icon
    High-efficient KO
    These Cas9 stable cell lines have been used to construct the KO cell lines for various genes, gene KO efficiency 5-10 times improved.
  • icon
    Curated cells
    Selected cells, low passages, good cell condition, high activity, applicable for all kinds of gene-editing experiments.
Related Resources: Red Cotton Gene-editing System | ATCC | DSMZ | ECAZZ | Cellosaurus | CCTCC | NCBI
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