PK-15-CAS9 cell line

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PK-15-CAS9 Order

PK-15-CAS9 cell line
Catalog#
YC-A017-Cas9-H
Size
1*10^6
Storage conditions
Shipped on dry ice; Store in liquid nitrogen
Datasheet
PK-15-CAS9 cell line
Order

Detailed Product Information

Product category
Cas9 Stable Cell Lines
cell name
PK-15-CAS9
Cell Morphology
Epithelial-like, adherent
Passage ratio
1：2-1：4
Species
Pig
Expression gene
Cas9
Resistance gene
hygro
Construction method
Lentivirus
Mycoplasma test
negative
Culture method
90%DMEM+10%FBS
Description
Use Cas9 gene expressing lentivirus constructed by Ubigene to infect the PK-15 cell line， screen the cells based on the resistance marker and the optimal antibiotic screening concentration explored in advance, resulting in a PK-15-CAS9 cell line that can maintain long-term, stable and high expression.
Product Validation Data（RT-QPCR）
Sample Name Target Name Cт Mean ΔCт
PK-15-CAS9 Cas9 21.41878700 4.06616783
PK-15-CAS9 β-actin 17.35261917
PK-15 Cas9 29.50979614 11.11094475
PK-15 β-actin 18.39885139

Product Advantage

  • Easy to use：Cas9 cell lines in our cell bank can stably express Cas9 protein. Each Cas9 Stable Cell Line is easy to use and enables gene knockout simply by transfecting gRNA, while transfection of gRNA and donor DNA results in gene knock-in or point mutations
    01
  • High-efficient KO：These Cas9 stable cell lines have been used to construct the KO cell lines for various genes, gene KO efficiency 5-10 times improved.
    02
  • High-quality cell：Selected cells, low passages, good cell condition, high activity, applicable for all kinds of gene-editing experiments.
    03
PK-15-CAS9 cell line
PK-15-CAS9
Catalog#
YC-A017-Cas9-H
Size
1*10^6
Storage conditions
Shipped on dry ice; Store in liquid nitrogen
Order

Related Popular Products

KO Cell Lines

  • VAMP2 Knockout cell line (PK-15)

    VAMP2 Knockout cell line (PK-15)
      inquiry   More details

    Organism: Pig
    Gene Name: VAMP2
    Gene ID: 100620549
    Size: 1*10^6
    Catalog#: YKO-R060
    YKO-R060(PK-15)
  • EEF1B2 Knockout cell line (PK-15)

    EEF1B2 Knockout cell line (PK-15)
      inquiry   More details

    Organism: Pig
    Gene Name: EEF1B2
    Gene ID: 100154920
    Size: 1*10^6
    Catalog#: YKO-R053
    YKO-R053(PK-15)
  • RIPK3 Knockout cell line (PK-15)

    RIPK3 Knockout cell line (PK-15)
      inquiry   More details

    Organism: Pig
    Gene Name: RIPK3
    Gene ID: 100153263
    Size: 1*10^6
    Catalog#: YKO-R051
    YKO-R051(PK-15)

Wild-type Cell Lines

Related Popular Products

KO Cell Lines

  • VAMP2 Knockout cell line (PK-15)

    VAMP2 Knockout cell line (PK-15)

    Organism: Pig
    Gene Name: VAMP2
    Gene ID: 100620549
    Size: 1*10^6
    Catalog#: YKO-R060
      inquiry   More details
  • EEF1B2 Knockout cell line (PK-15)

    EEF1B2 Knockout cell line (PK-15)

    Organism: Pig
    Gene Name: EEF1B2
    Gene ID: 100154920
    Size: 1*10^6
    Catalog#: YKO-R053
      inquiry   More details
  • RIPK3 Knockout cell line (PK-15)

    RIPK3 Knockout cell line (PK-15)

    Organism: Pig
    Gene Name: RIPK3
    Gene ID: 100153263
    Size: 1*10^6
    Catalog#: YKO-R051
      inquiry   More details

Wild-type Cell Lines

PK-15-CAS9 Order

PK-15-CAS9 cell line
Catalog#
YC-A017-Cas9-H
Size
1*10^6
Storage conditions
Shipped on dry ice; Store in liquid nitrogen
Datasheet
PK-15-CAS9 cell line
Order

Detailed Product Information

Product category
Cas9 Stable Cell Lines
cell name
PK-15-CAS9
Cell Morphology
Epithelial-like, adherent
Passage ratio
1：2-1：4
Species
Pig
Expression gene
Cas9
Resistance gene
hygro
Construction method
Lentivirus
Mycoplasma test
negative
Culture method
90%DMEM+10%FBS
Description
Use Cas9 gene expressing lentivirus constructed by Ubigene to infect the PK-15 cell line， screen the cells based on the resistance marker and the optimal antibiotic screening concentration explored in advance, resulting in a PK-15-CAS9 cell line that can maintain long-term, stable and high expression.
Product Validation Data（RT-QPCR）
Sample Name Target Name Cт Mean ΔCт
PK-15-CAS9 Cas9 21.41878700 4.06616783
PK-15-CAS9 β-actin 17.35261917
PK-15 Cas9 29.50979614 11.11094475
PK-15 β-actin 18.39885139

Product Advantage

  • Easy to use：Cas9 cell lines in our cell bank can stably express Cas9 protein. Each Cas9 Stable Cell Line is easy to use and enables gene knockout simply by transfecting gRNA, while transfection of gRNA and donor DNA results in gene knock-in or point mutations
    01
  • High-efficient KO：These Cas9 stable cell lines have been used to construct the KO cell lines for various genes, gene KO efficiency 5-10 times improved.
    02
  • High-quality cell：Selected cells, low passages, good cell condition, high activity, applicable for all kinds of gene-editing experiments.
    03
PK-15-CAS9 cell line
PK-15-CAS9
Catalog#
YC-A017-Cas9-H
Size
1*10^6
Storage conditions
Shipped on dry ice; Store in liquid nitrogen
Order

Related Popular Products

KO Cell Lines

  • VAMP2 Knockout cell line (PK-15)

    VAMP2 Knockout cell line (PK-15)
      inquiry   More details

    Organism: Pig
    Gene Name: VAMP2
    Gene ID: 100620549
    Size: 1*10^6
    Catalog#: YKO-R060
    YKO-R060(PK-15)
  • EEF1B2 Knockout cell line (PK-15)

    EEF1B2 Knockout cell line (PK-15)
      inquiry   More details

    Organism: Pig
    Gene Name: EEF1B2
    Gene ID: 100154920
    Size: 1*10^6
    Catalog#: YKO-R053
    YKO-R053(PK-15)
  • RIPK3 Knockout cell line (PK-15)

    RIPK3 Knockout cell line (PK-15)
      inquiry   More details

    Organism: Pig
    Gene Name: RIPK3
    Gene ID: 100153263
    Size: 1*10^6
    Catalog#: YKO-R051
    YKO-R051(PK-15)

Wild-type Cell Lines

Related Popular Products

KO Cell Lines

  • VAMP2 Knockout cell line (PK-15)

    VAMP2 Knockout cell line (PK-15)

    Organism: Pig
    Gene Name: VAMP2
    Gene ID: 100620549
    Size: 1*10^6
    Catalog#: YKO-R060
      inquiry   More details
  • EEF1B2 Knockout cell line (PK-15)

    EEF1B2 Knockout cell line (PK-15)

    Organism: Pig
    Gene Name: EEF1B2
    Gene ID: 100154920
    Size: 1*10^6
    Catalog#: YKO-R053
      inquiry   More details
  • RIPK3 Knockout cell line (PK-15)

    RIPK3 Knockout cell line (PK-15)

    Organism: Pig
    Gene Name: RIPK3
    Gene ID: 100153263
    Size: 1*10^6
    Catalog#: YKO-R051
      inquiry   More details

Wild-type Cell Lines


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